Hi Greg:  I just asked my supervisor the very same question (sort of).  She
told me to air dry the frozen sections overnight.  They can then be stored
at -70 F for up to 6 months.  Mind you, I'm doing 10 micron brain sections.
Hope this helps.  Judi

> -----Original Message-----
> From:	Greg Dobbin [SMTP:dobbin@Upei.CA]
> Sent:	Wednesday, July 19, 2000 7:38 AM
> To:	Histonet@pathology.swmed.edu
> Subject:	Revisiting storage of cryosections
> 
> Hello Histonetters,
> I do recall this subject being discussed before, but at the time I 
> read with passing interest. I now have a graduate student 
> wondering how long an air-dried frozen section can be stored and 
> under what conditions, prior to doing immunoelectron microscopy.
> He would like to batch process his sections. Any suggestions?
> Cheers! Greg
> >
> >
> >
> >
> >
> >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 Unviversity Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851