RE:Re: RE:carazzi's Hematoxylin
<< Previous Message | Next Message >>
From: | Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt> |
To: | histonet@pathology.swmed.edu, Tom Wells <tomers@home.com> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
to Tom Wells,
Carazzi's Hx.
Hx. 0.5g
Glycerol 100 cc
Potassium alum 25g
distilled water 400cc
Potassium iodate 0.1g
Overnight, dissolve the Hx. in the glycerol, and the alum in most of the water.
Next morning, the alum solution is added slowly to the Hx solution, mixing very
well after each addition. The potassium iodate is dissolved in the rest of the
water, with gentle warming, and then added to the Hx mixture above.
The final staining solution is shaken very well and is ready for immediate use.
Shelf life - 6 months
Wherever possible, it is safer to dissolve reagents without using heat, to avoid
over-oxidation
Carazzi's may be used progressively, as one would need for IHC or regressively
followed by differentiation and blueing.
Good luck
Carmen
St. Luke's
Malta
<< Previous Message | Next Message >>