My "Harleco Schiff Reagent, lot 1030" still works in the Feulgen reaction.
I got it from EM Diagnostic Systems, Gibbstown, NJ 08027, a while ago. They
might be selling different stuff now.
	The Feulgen reaction is sensitive to fixation.  Have you changed
fixatives?
				Allen A. Smith
				School of Graduate Medical Sciences
					Podiatric Medicine and Surgery
				Barry University
				Miami Shores, Florida

			-----Original Message-----
From: CMCCOLLOUGH@dnr.state.md.us [mailto:CMCCOLLOUGH@dnr.state.md.us]
Sent: Monday, July 17, 2000 2:11 PM
To: histonet@pathology.swmed.edu
Subject: Need supplier of Schiff's stain for Feulgen reaction


Hello Histonetters:

I am tearing my hair out.  I have attempted to make Schiff's that will work
in the Feulgen reaction (a routine stain in our lab) using 4 different lots
of dry stain and 3 different recipes (Lilllie's cold, Coleman's hot, and
Bancroft & Stevens), all to no avail.  Previously wonderful ready-made stain
from our usual supplier no longer works (have tried 4 or 5 different lots).
This supplier has recently changed lots of dry stain as well.  I have tried
a second ready made stain to no avail.  All work fine in the PAS stain, but
not Feulgen.

Is there a vendor who can supply me with a Schiff's tested for Feulgen?

Comments from John Kiernan, Samurai, and other Histonet gurus are most
welcome.

Regards -
Carol
*****************
Carol B. McCollough, HT(ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Fisheries Service
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, MD 21654