RE: Kappa and Lambda IHC

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We currently retrieve Lambda 30 minutes in steamer, and use protease on 
Kappa.  We used to have a lot of trouble with background staining on both 
slides, but this has pretty much cleaned that up.  We also treat slides 
before staining in 3% hydrogen peroxide for 10 minutes.  Hope this helps

Jennifer Macias, HT (ASCP)
Forum Health 
Western Reserve Care System
Youngstown, Ohio

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