RE: Kappa and Lambda IHC

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From:"Goodwin, Diana" <DGoodwin@CHSNJ.org>
To:'HistoNet' <HistoNet@Pathology.swmed.edu>, "'Trivett Melanie (PMC)'" <TrivettMelanie@petermac.unimelb.edu.au>
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Dear Melanie,

I am using Dako concentrates for K and L diluted to 1:2000 with tonsil
as control and no pretreatment with good results.


Diana Goodwin,  HT
Trenton,  NJ


> ----------
> From: 	Trivett Melanie
> (PMC)[SMTP:TrivettMelanie@petermac.unimelb.edu.au]
> Sent: 	Wednesday, July 26, 2000 2:40 AM
> To: 	'HistoNet'
> Subject: 	Kappa and Lambda IHC
> 
> Hello all,
> 
> Was wondering if any kind souls out there could give me some advice on
> Kappa
> and Lambda staining on formalin fixed paraffin embedded human tissue.
> Currently we use tonsil as a control and use HIER by pressure cooking
> for 2
> minutes in citrate buffer (pH 6.0).  At the moment we get some
> background
> staining.  I have heard that some people use enzyme digestion for
> these
> antibodies...any comments??
> 
> Thanks in advance.
> 
> Melanie Trivett
> Peter MacCallum Cancer Institute
> Melbourne
> Australia
> 



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