RE: Kappa and Lambda IHC
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From: | "Goodwin, Diana" <DGoodwin@CHSNJ.org> |
To: | 'HistoNet' <HistoNet@Pathology.swmed.edu>, "'Trivett Melanie (PMC)'" <TrivettMelanie@petermac.unimelb.edu.au> |
Reply-To: | |
Content-Type: | text/plain |
Dear Melanie,
I am using Dako concentrates for K and L diluted to 1:2000 with tonsil
as control and no pretreatment with good results.
Diana Goodwin, HT
Trenton, NJ
> ----------
> From: Trivett Melanie
> (PMC)[SMTP:TrivettMelanie@petermac.unimelb.edu.au]
> Sent: Wednesday, July 26, 2000 2:40 AM
> To: 'HistoNet'
> Subject: Kappa and Lambda IHC
>
> Hello all,
>
> Was wondering if any kind souls out there could give me some advice on
> Kappa
> and Lambda staining on formalin fixed paraffin embedded human tissue.
> Currently we use tonsil as a control and use HIER by pressure cooking
> for 2
> minutes in citrate buffer (pH 6.0). At the moment we get some
> background
> staining. I have heard that some people use enzyme digestion for
> these
> antibodies...any comments??
>
> Thanks in advance.
>
> Melanie Trivett
> Peter MacCallum Cancer Institute
> Melbourne
> Australia
>
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