You might also like to check your pH and make sure it is at 6.0 +/- 0.2, as
we have found ER very sensitive to the citrate buffer pH changes.
b

> -----Original Message-----
> From:	a i d a n   s c h u r r [SMTP:Aidan.Schurr@hvh.co.nz]
> Sent:	July 19, 2000 10:51 PM
> To:	carolyn.luty@muhc.mcgill.ca; histonet@pathology.swmed.edu
> Subject:	Re: ER
> 
> Carolyn,
> 
> My guess would be that the antigen retrieval is to blame.  We had 
> a problem like this recently - I discovered the citrate wasn't getting 
> hot enough to retrieve efficiently.  Have a play with that and see if it 
> helps!!
> 
> Good luck
> Aidan
> > 
> > We are having problems with very weak staining with ER. We use antigen
> retrieval
> > in citrate buffer in the microwave.The antibody was from Nova-Castra and
> then we
> > tried one from Biogenex with the same results. Can anyone give any
> suggestions
> > ??.
> > 
> > Carolyn Luty
> > Montreal General Hospital
> > 
> > 
> > 
> 
> 
> ___________________________________________________
> shin: device for finding furniture in the dark...
> ___________________________________________________
> a i d a n   c   s c h u r r 
>      mlso,  histology department
>       hutt valley health
>        lower hutt, new zealand
> 
>      ph.  ++64 4 5709173
>      fax  ++64 4 5709214
> ___________________________________________________
>