RE: ER

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From:powell_sa <powell_sa@Mercer.EDU>
To:carolyn.luty@muhc.mcgill.ca, histonet <histonet@pathology.swmed.edu>
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Content-Type:text/plain; charset=ISO-8859-1

I use NovaCastra's ER (clone 6F11) and have good results, I steam retrieve at 
present, (I find it more gentle) but have done the microwave, and am getting a 
Decloaking chamber (pressure cooker)from BioCare.  The one thing I have 
learned is that ER/PR are heat sensitive, ER more so than PR, and works better 
on the counter than on my NexES.  I also have used Dako's ER, Zymed's ER, and 
Biogenex's ER, all of which have worked well for me, but if you want to talk 
live, give me a call at 1-912-301-2374 to discuss your procedure.

Shirley Powell


>===== Original Message From carolyn.luty@muhc.mcgill.ca =====
>We are having problems with very weak staining with ER. We use antigen 
retrieval
>in citrate buffer in the microwave.The antibody was from Nova-Castra and then 
we
>tried one from Biogenex with the same results. Can anyone give any 
suggestions
>??.
>
>Carolyn Luty
>Montreal General Hospital




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