Hi Denise,
We use the DAKO immunostainer with Sigma's 10mM PBS ph 7.4.     We switched
from DAKO's buffer because the Sigma was a lot cheaper.    We do use the
DAKO antibody diluent.
Hazel

> -----Original Message-----
> From:	denise M m Long-Woodward [SMTP:denisew2@juno.com]
> Sent:	Monday, July 24, 2000 05:03 AM
> To:	histonet@pathology.swmed.edu
> Subject:	Dako autostainer
> 
> I've been asked to relay a question to all those who work with Dako's
> Autoimmunostainer. What are you using for Buffer rinses: Dako's
> commercial  TBS or something else?  Those who use something else, could
> you please tell me what it is?   They also want to know what you are
> using to dilute your antibodies. Are you using Dako's commerical stuff?
> Again please specify the diluent being used. 
> 
> Many thanks for your help!
> 
> Denise Long Woodward
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