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From:"Jennings-Siena, Debbie" <>
To:'william thoendel' <>,

WE do three levels and a cytokeratin stain on all negative sentinel lymph
Debbie J. Siena, HT(ASCP)
Histopathology Manager
Baylor University Medical Center
Dallas, TX 75246
214-820-2465 vm
214-820-4110 fax

-----Original Message-----
From: william thoendel []
Sent: Wednesday, July 26, 2000 5:04 PM

Hello Netters,
What are the protocols used for Sentinel Node sectioning in the histology 
labs?  We initially cut three H&E levels of the tagged lymph node(s) and 
save a slide for possible IHC staining.  If the inital levels are negative 
for tumor seven additional levels are cut with additional slides from these 
levels saved for possible IHC staining.  What additional testing is 
performed in your labs?
Thanks in advance,
Bill Thoendel
Pathology Medical Services
Norfolk, Nebraska
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