Practical methods for cartilage

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From:Jerry Chi-Yuan Hu <>
To:HistoNet Server <>
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Hello Everyone,

I've noticed several messages in the archive about decalcifying cartilage,
but I would also like to know how decalcifying affects the non-calcified
cartilage.  I would like to compare the shapes of chondrocytes from the
various zones of fresh cartilage, and I need the cell shape to be
preserved a well as possible.  Does anyone have a preferred method to
process fresh cartilage, down to the calcified zone, that they can send to
me?  Preferred stains to visualize cell shape?  Any help will be greatly

Jerry Hu

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