Practical methods for cartilage

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From:Jerry Chi-Yuan Hu <ragnarok@ruf.rice.edu>
To:HistoNet Server <histonet@pathology.swmed.edu>
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Hello Everyone,

I've noticed several messages in the archive about decalcifying cartilage,
but I would also like to know how decalcifying affects the non-calcified
cartilage.  I would like to compare the shapes of chondrocytes from the
various zones of fresh cartilage, and I need the cell shape to be
preserved a well as possible.  Does anyone have a preferred method to
process fresh cartilage, down to the calcified zone, that they can send to
me?  Preferred stains to visualize cell shape?  Any help will be greatly
appreciated.

Jerry Hu




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