Kappa and Lambda IHC

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From:"Trivett Melanie (PMC)" <TrivettMelanie@petermac.unimelb.edu.au>
To:'HistoNet' <histonet@pathology.swmed.edu>

Hello all,

Was wondering if any kind souls out there could give me some advice on Kappa
and Lambda staining on formalin fixed paraffin embedded human tissue.
Currently we use tonsil as a control and use HIER by pressure cooking for 2
minutes in citrate buffer (pH 6.0).  At the moment we get some background
staining.  I have heard that some people use enzyme digestion for these
antibodies...any comments??

Thanks in advance.

Melanie Trivett
Peter MacCallum Cancer Institute

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