Kappa & Lambda

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From:rzumbo <rzumbo@iofm.cs.nsw.gov.au>
To:HISTONET@pathology.swmed.edu
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Hi Melanie,
We use the following with very good results.
DAKO antibodies and DAKO LSAB kit

Kappa - 1:4000 dilution
Hydrogen Peroxide - 5 min
Protease K - 3-5min
Kappa Antibody - 10min
And follow as per kit times


Lambda - 1:5000 dilution
Hydrogen Peroxide - 5 min
Protease K - 3-5min
Lambda Antibody - 10min
And follow as per kit times

Hope it helps

Rosalba Zumbo
Technical Officer ( Scientific)
Institute of Forensic Medicine
Sydney, Australia



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