Kappa & Lambda
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From: | rzumbo <rzumbo@iofm.cs.nsw.gov.au> |
To: | HISTONET@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Hi Melanie,
We use the following with very good results.
DAKO antibodies and DAKO LSAB kit
Kappa - 1:4000 dilution
Hydrogen Peroxide - 5 min
Protease K - 3-5min
Kappa Antibody - 10min
And follow as per kit times
Lambda - 1:5000 dilution
Hydrogen Peroxide - 5 min
Protease K - 3-5min
Lambda Antibody - 10min
And follow as per kit times
Hope it helps
Rosalba Zumbo
Technical Officer ( Scientific)
Institute of Forensic Medicine
Sydney, Australia
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