Fwd: Revisiting storage of cryosections

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From:Karen Larison <larisonk@uoneuro.uoregon.edu>
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Cryosections yield very poor quality immunoelectron micrographs 
because of ice crystal damage.  It's my understanding that a better 
method is to use vibratome sections,  If you do your 
immunohistochemistry on free-floating vibratome sections,they can be 
washed, dehydrated and embedded in the EM resin of your choice.

Karen in Oregon

>Date: Wed, 19 Jul 2000 11:37:31 -0300 (ADT)
>From: Greg Dobbin <dobbin@Upei.CA>
>Subject: Revisiting storage of cryosections
>To: Histonet@pathology.swmed.edu
>Hello Histonetters,
>I do recall this subject being discussed before, but at the time I
>read with passing interest. I now have a graduate student
>wondering how long an air-dried frozen section can be stored and
>under what conditions, prior to doing immunoelectron microscopy.
>He would like to batch process his sections. Any suggestions?
>Cheers! Greg
>Greg Dobbin
>Pathology Lab
>Atlantic Veterinary College, U.P.E.I.
>550 Unviversity Ave.
>Charlottetown, P.E.I.
>Canada,  C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851

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