Carolyn

We use DAKO monoclonal ER with antigen retrieval in DAKO TRS by way of
microwave with Imidazole enhancement. We get very good results.

Jim Manavis
ICC
IMVS
-----Original Message-----
From: carolyn.luty@muhc.mcgill.ca <carolyn.luty@muhc.mcgill.ca>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Thursday, 20 July 2000 15:37
Subject: ER


>
>
>We are having problems with very weak staining with ER. We use antigen
retrieval
>in citrate buffer in the microwave.The antibody was from Nova-Castra and
then we
>tried one from Biogenex with the same results. Can anyone give any
suggestions
>??.
>
>Carolyn Luty
>Montreal General Hospital
>
>
>
>