Boneheads - plastic info

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From:Gayle Callis <>
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Caveat - The comment about using Technovits in a Shandon processor - I
believe that the heating cycle or some type of modification is required to
put any plastics (PMMA) in this processor must be done BEFORE you try this.
 You need to contact the Shandon people BEFORE putting methylmethacrylate
in this machine. 

>Date: Fri, 28 Jul 2000 15:20:04 -0400
>From: Linda Jenkins <>
>Subject: Boneheads - plastic info
>Hi, Linda!
>	Well...I based my volume of monomer on the "fixative standard".
>That is, 15 -20 volumes of monomer for every volume of tissue.  Now this is
>not a scientific study - but it seems to work well for me.  Yes, Technovit
>7200 is pricey but I can reuse it MANY times by using the following
>1. Place  up to 30 cassettes in large glass desiccator.  Add 2 - 3 bottles
>of Technovit 7200(enough to cover the specimens with 2-3 inches of monomer.
>Add a large magnetic stir bar and attach vacuum lid.
>2.  Place desiccator on magnetic stir plate.
>3.  Attach desiccator to vacuum and pull vacuum until you see bubbles rise
>from the surface of the monomer.
>4. Turn on magnetic stirrer to a moderate speed.
>5.  Cover ENTIRE desiccator with a light proof cloth.  ***This, of course,
>keeps the light-curing Technovit 7200 from polymerizing.
>6.  For cross sections of undecalcified goat femur, I infiltrate for seven
>days, changing the solution once after 48 hours.
>7.  The Technovit 7200 is filtered and returned to its original brown
>bottle and labeled with the date of its use.
>	I have some solutions that are almost two years old and
>infiltrating just fine!  The best part is when you polymerize with the
>Technovit 7200 in 8 hours instead of 2 days to 2 weeks with
>	I also heard that some Canadian people were using the Technovit
>7200 in the Shandon automatic processor with fantastic results.
>	Hope this helps!
>	Linda
>Linda Jenkins, HT
>Clemson University
>Department of Bioengineering
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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