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From: | Mike King <making@nersp.nerdc.ufl.edu> |
To: | histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Jerry Chi-Yuan Hu [mailto:ragnarok@ruf.rice.edu] wrote: "....I am looking
for an embedding medium that is not toxic and hardens to an appreciable
stiffness above 0oC..."
There is an albumin-gelatin method that uses glutaradehyde to set; it
produces a stiff rubbery gel that cuts pretty well frozen or with a
vibratome. There is some toxicity from the glut but standard precautions
deal with it. Reply if this might be of interest to you and I'll try to
find the details--we haven't used it in almost 20 years and I can't put my
hands on it right now. Maybe another reader uses it?
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