recovering endogenous biotin with antigen retrieval, casein blockers -Reply

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From:Richard Cartun <>,,
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Now that we are using "extreme" heat retrieval pretreatments, I am finding endogenous biotin everywhere (not just the traditional kindey. liver, thyroid, brain).  Therefore, I have switched almost all of our IP procedures over to a non-avidin/biotin detection system.

R. Cartun

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