processing bugs

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From:Andrea Grantham <algranth@U.Arizona.EDU> (by way of histonet)
To:histonet@histosearch.com
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Hello Histonetters,
I was just asked to start processing whiteflies for in situ detection of
intracellular symbiotic bacteria.
Two questions -
Is anybody processing bugs? I'm looking for a protocol.
My usual processing protocol for animal tissue is one hour each in
increasing strengths of ETOH starting with 70% and going to Paraplast Xtra.
Total processing time is 12 hours. Is this too long for insect processing?
Thanks.
Andi


.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html




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