processing bugs
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From: | Andrea Grantham <algranth@U.Arizona.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello Histonetters,
I was just asked to start processing whiteflies for in situ detection of
intracellular symbiotic bacteria.
Two questions -
Is anybody processing bugs? I'm looking for a protocol.
My usual processing protocol for animal tissue is one hour each in
increasing strengths of ETOH starting with 70% and going to Paraplast Xtra.
Total processing time is 12 hours. Is this too long for insect processing?
Thanks.
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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