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From:"Sebree Linda A." <la.sebree@hosp.wisc.edu>
To:'Histonet' <histonet@pathology.swmed.edu>

Hi 'netters,

Does anyone out there have a protocol for p21 (also designated WAF1/Cip1)
for use on FFPE tissue; preferably on a Ventana automated immunostainer?

I have Santa Cruz's antibody (cat. #sc-8170) and they (SC) recommended HIER
with citrate buffer, 1:50 to 1:100 dilution and colon CA as a positive
control.  My first attempt yielded negative results and as this is a
research project, all the optimization time and cost is borne by the
researcher and I'm trying to keep his costs down by not reinventing the

Thanks in advance,

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
600 Highland Avenue
Madison, WI  53792-2472

FAX: (608)263-1568

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