cytokine IHC again

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To any cytokine stainers:

Last week put out a kind of theory question (as opposed to purely
procedural) re: use (or necessiity) of saponin in IHC washes on frozens and
paraffin when looking for cytokines.  Don't know if it made it out on the
Net as I've not seen a single response.
Have talked to people who say it is essential.  My cytokines seem to stain
with or without saponin.  Any ideas on the reasoning behind the saponin?

Thanks for any thoughts.

Immunex Corp.
Seattle, WA

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