cryostat frost build up woes

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From:Gayle Callis <>
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In Montana, land of little humidity, we suffer from same frost buildup in
our Cryocut 1800 as you do.   We live with it, and defrost/clean cryostat
more often.  I get a glacier of ice build up in the back of microtome big
enough to cause the moving parts of microtome to bump into it. High usage
is the biggest problem with steamy hands, etc in the machine.  We try to
minimize keeping the sliding door open when not working in the chamber.   

One thing we have done is make sure the fans, etc on innards of cryostat
are free of dust.  DO THIS DURING CLEANING OF MACHINE. Take back off the
cryostat AFTER UNPLUGGING and blow dust out with compressed air.  We had
temperature flucuations one time, but do this about once a year to insure
cooling (as with refrigerators!) is not impeded with dust bunny build up.    


Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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