Hello Everyone,

I am looking for a way to isolate cells from thin slices (at 100 um 
thick) and to start a cell line from these cells.  Everyone I've spoken to 
says that the fresh tissue has to be embedded for slicing in a microtome 
cryostat, though slices of 100 um might crack...  I also do not know if 
the embedding medium is going to kill my cells.  

If anyone knows how to use the microtome (or cryotome) to get thick slices
of live cells please let me know.  Your help is greatly appreciated!

Jerry Hu