Re: sections

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From:Taffy <>
To:Monique Tourand <>,
Content-Type:text/plain; charset=us-ascii

I have been beating myself up over the SAME problem..the mice feet will NOT
section..I thought they were not completely fixed..I have shortened the
processing time, I have extended the processing time, we have used EDTA to
decal as well as Decal I..nothing seems to work. I keep getting the same
kind of sections that you describe. Let me know if you find the answer and I
will do likewise.
Taffy Hooser

At 09:05 AM 6/29/00 -0700, you wrote:
>Hello there,
>I just joined the histonet so this will be very helpful to me.  I 
>received two mice feet to embed and cut, they were fixed then I 
>decalcified them with Cal-ex.  I processed them as usual and blocked 
>them, but now when I tried to cut them they won't cut, there is just 
>a hole in the paraffin sections where the sample is.  When I cut it 
>sounds as if the bone is still a bit hard so I soaked the blocks 
>again in Cal-ex to see if that would help and I haven't yet tried to 
>cut them again.  Does anyone esle have any other suggestions that 
>will help or is there some other trick for cutting bone this is the 
>first time I have had to do it.  Thanks for any help.
>Monique Tourand
>University of Alberta
Mary (Taffy) Hooser, HT(ASCP)
Department of Anatomy and Cell Biology
Indiana University School of Medicine
MS-5035, 635 Barnhill Drive
Indianapolis, IN. 46202-5120
Tel: (317)274-2308.
Fax: (317)278-2040.

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