I can't say that I've processed any bugs successfully, however I have 
processed a fly on a 12 hour routine process - it was completely over 
processed, I would definitely take off all heat from the reagents and back 
way off the alcohol.  Not sure what type of fixative to use.
Stacey
Conway Medical Regional Center
Conway AR

>From: Andrea Grantham <algranth@U.Arizona.EDU>
>To: histonet@pathology.swmed.edu
>Subject: processing bugs
>Date: Thu, 06 Jul 2000 12:00:59 -0700
>
>Hello Histonetters,
>I was just asked to start processing whiteflies for in situ detection of
>intracellular symbiotic bacteria.
>Two questions -
>Is anybody processing bugs? I'm looking for a protocol.
>My usual processing protocol for animal tissue is one hour each in
>increasing strengths of ETOH starting with 70% and going to Paraplast Xtra.
>Total processing time is 12 hours. Is this too long for insect processing?
>Thanks.
>Andi
>
>
>.....................................................................
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>: Sr. Research Specialist       University of Arizona               :
>: (office:  AHSC 4212)          P.O. Box 245044                     :
>: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
>: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
>:...................................................................:
>           http://www.cba.arizona.edu/histology-lab.html
>

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