Re:p21 protocol

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         We use the CalBiochem brand of p21 at a dilution of 1:50 with a colon
carcinoma as a positive control and it works very well on FFPE sections.  An
antigen retrieval time of 25 minutes using a steamer and the Dako target ret.
solution.  Primary antibody incubation of 1 hour at room temp with the
LSAB+ kit
by dako for the rest.  Good luck to you.

Sincerely yours,
Chrisitne Klein
MD Anderson

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