Re:p21 protocol
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From: | cklein@mail.mdanderson.org (by way of histonet) |
To: | histonet@histosearch.com |
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Linda,
We use the CalBiochem brand of p21 at a dilution of 1:50 with a colon
carcinoma as a positive control and it works very well on FFPE sections. An
antigen retrieval time of 25 minutes using a steamer and the Dako target ret.
solution. Primary antibody incubation of 1 hour at room temp with the
LSAB+ kit
by dako for the rest. Good luck to you.
Sincerely yours,
Chrisitne Klein
MD Anderson
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