Oncogene Sciences p21 (WAF1/Cip1) Clone EA10.  10 minute microwave 0.01M
Citrate pH 6.0 + Protease 1 for 2 minutes (Protease alone works well also) 1:20
for 32 minutes

"Sebree Linda A." wrote:

> Hi 'netters,
>
> Does anyone out there have a protocol for p21 (also designated WAF1/Cip1)
> for use on FFPE tissue; preferably on a Ventana automated immunostainer?
>
> I have Santa Cruz's antibody (cat. #sc-8170) and they (SC) recommended HIER
> with citrate buffer, 1:50 to 1:100 dilution and colon CA as a positive
> control.  My first attempt yielded negative results and as this is a
> research project, all the optimization time and cost is borne by the
> researcher and I'm trying to keep his costs down by not reinventing the
> wheel.
>
> Thanks in advance,
>
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
>
> (608)265-6596
> FAX: (608)263-1568

--
Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016
(212)562-4667