In the past we have used the TRAP assay (Sigma) to look at osteoclasts in
10% formalin fixed mouse knees, decalcified ( 5% EDTA) paraffin sections.
Gina




                    "Gaskill, Betty

                    A"                       To:
Histonet@pathology.swmed.edu
                    <bg11802@glaxowel        cc:

                    lcome.com>               Subject:     osteoclast
staining


                    07/06/00 02:58 PM








Anybody out there have an antibody they are using for osteoclast staining
in
10% formalin fixed, decalcified, paraffin sections?  Would love to know of
antibodies, protocols any info would be greatly appreciated.
Thanks
Betty