Re: osteoclast staining
<< Previous Message | Next Message >>
From: | hborgeri@wfubmc.edu (Hermina Borgerink) (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Betty,
Here is a reference for a histochemical TRAP procedure that will stain
osteoclasts beautifully in formalin fixed, demineralized, and paraffin
embedded bone: Erlebacher and Derynck, J. Cell Biol. 132: 195-210,
1996. The only thing I modified was the incubation time, which I
increased from one hour to three to four hours. I used Mayer's
hematoxylin as a counterstain.
Hermina Borgerink
Wake Forest University
Department of pathology
Winston-Salem, NC
"Gaskill, Betty A" wrote:
>
> Anybody out there have an antibody they are using for osteoclast staining in
> 10% formalin fixed, decalcified, paraffin sections? Would love to know of
> antibodies, protocols any info would be greatly appreciated.
> Thanks
> Betty
<< Previous Message | Next Message >>