Re: frosty cryostat

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From:Becky Scholes <>,
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So, you have a 2800N, too!  We've noticed that any cryostat will frost up in 
a humid climate.  Ideally, if you have the space, you need a dehumidifier in 
the room.  Leica suggested that if you are in a sunny room, to pull the 
shades.  I live in swampy Iowa where the summer humidity is "good for the 
corn".  We are in a sunny room, and pull the shades and crank on the air 
conditioner.  We also defrost the unit once a week.  One of my techs does 
Mohs' on a Tissue-Tek II all day, and she will turn on the defrost cycle 
during the noon break to clean out some of the frost.  I have not had to be 
that drastic with the Frigocut, but give the defrost cycle a try.  Then 
adjust the cycle for another run as usual during the night.

Bec the Tec

>Subject: frosty cryostat
>Date: Sat, 01 Jul 2000 12:50:40 -0400 (EDT)
>Fellow Histonetters,
>This question is directed mostly to those techs who live in humid areas and
>do lots of frozens in old labs which are not "climate controlled".
>Usually Tucson has a very dry climate but during the summer monsoons it 
>a little swampy. We are in the monsoon season now and the rain is great for
>most things but not for my cryostat.
>My cryostat, a Leica 2800N, has been frosting up something awful because it
>is so humid in our room. I put a tray of drierite in the chamber and it
>turned pink almost immediately. Of course it doesn't help to be cutting
>sections and having the window open for long stretches of time. Does 
>have any tips on preventing the frost build up in a normally frost free
>Andi Grantham
>University of Arizona
>   =^..^=

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