Re: avidin/biotin blocking in house kit
<< Previous Message | Next Message >>
From: | Phyllis Davie <pdavie@phenopath.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Gayle,
I was interested in your procedure for in-house avidin/biotin blocking.
I looked in the sigma catalog, and there are several forms and grades of
avidin and streptavidin available. Do you have a catalog number? I only
saw 1 entry for d-biotin, cat# B4501. Is that the one you use?
Thanks for your help.
>avidin D 0.01% in PBS, available from Sigma
>
>d-biotin 0.005% in PBS, available from Sigma
>
>You could probably add sodium azide 10 - 15 mM to the PBS to preserve the
>solutions
>
>avidin solution 15 - 20 min
>rinse well
>biotin solution 15 - 20 min
>rinse well
>
>Times for avidin and biotin block and rinses can be extended if desired,
>and dependent on the IHC method (seeing differences in some protocols)
>Gayle Callis
>Veterinary Molecular Biology
>Montana State University
>Bozeman MT 59717-3610
>406 994-4705
>406 994-4303
>
Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
3000 First Ave., #1
Seattle, WA 98121
pdavie@phenopath.com
(206)374-9009 phone
(206)374-9009 fax
www.phenopath.com
<< Previous Message | Next Message >>