Re: Using Mineral Oil instead of Xylene in processing tissue
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Mary Stevens <Mstevens@genetics.com> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 10 Jul 2000, Mary Stevens wrote:
> There was a paper in 1992 - Vegetable Oils instead of Xylene in Tissue Processing", APMIS 100:827-83l which may make interesting reading for those of you interested in making the transition to oils.
> Mary
Thanks very much. I'll look it up. Veggie oils are a bit like the
thicker mineral ones: mix with xylene or wax or higher alcohols,
but not (or not completely) with ethanol. Lillie's big book notes
the use of gasolene as a clearing agent in wartime. It had to be
fuel for aeroplanes (not cars) for some reason - perhaps alcohol
miscibility. (Sorry, haven't got the book to hand right now.)
Essential oils, which do mix with ethanol, were used in the days
when it was impossible for a simple scientist to obtain ethyl
alcohol better than 95%. Some of the essential oils can carry
a specimen from 85% or so to paraffin wax. Cedarwood oil was and
still is generally considered the best. I think terpineol (synthetic
oil of lilac) is just as good and it's less expensive.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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