Re: Oops--wrong 2ndary--any hope?

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From:Katri Tuomala <>
To:Bret Morrow <>
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Hi Bret,
Depending on what the wrong secondary was, in what animal your primary was
rased and on what species of tissue you were working, the fix could be
simple or complicated. Give us more info.

Katri Tuomala
St.Joseph's Hospital
Hamilton, Ontario, Canada

Bret Morrow wrote:

> Greetings,
> We had a SNAFU and probed free-floating tissue with the right primary
> but the wrong 2ndary antibody and then ABC.  The tissue was NOT exposed
> to DAB chromogen.  Before we figured out what had happened, we washed
> the tissue and placed it into a cryo solution and stored it @ -20 oC.
> The tissue is reasonable valuable and is, of course, the last set of
> tissue slices from these animals.  Now that we know the wrong secondary
> was used, what are the odds that we can get any signal from the antigen
> target using the right secondary?  Could we re-expose to more primary
> and then the right secondary and get a good signal?   Is there concern
> that the signal may be diminished?   Any thoughts on this, anyone?
> ____________________________________________
> Bret A. Morrow, Ph.D.
> Associate research scientist
> Associate clinical professor
> Yale University School of Medicine
> New Haven, CT 06520-8066
> voice 203.785.4515
> fax  203.785.7670
> email

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