Re: Marker for T-cells in sheep

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From:"Pam M. Dilbeck" <>
To:Jenny Molde <>
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Jenny--I have run Dako's CD3 A452 on several different species (canine,
feline, equine, etc) but never tried on ovines.  You might try it--run a
lymph node as a control tissue.  I use citrate buffer with steam for
antigen retrieval and incuabate overnight in refrig. (use at 1:50,000).  I
use Signet's ultra streptavidin (USA) detection and AEC for the
chromogen.  Let me know if you would like more information.

On Tue, 11 Jul 2000, Jenny Molde wrote:

> Dear Histonetters
> I am looking for a lymphocyte marker for sheep. Anyone out there had any
> experience with this. Many thanks.
> Jenny Molde

	Pam Dilbeck
	Immunohistochemistry Section
	Washington Animal Disease Diagnostic Laboratory

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