Re: Marker for T-cells in sheep
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From: | "Pam M. Dilbeck" <pmd@vetmed.wsu.edu> |
To: | Jenny Molde <ctsmolde@samiot.uct.ac.za> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Jenny--I have run Dako's CD3 A452 on several different species (canine,
feline, equine, etc) but never tried on ovines. You might try it--run a
lymph node as a control tissue. I use citrate buffer with steam for
antigen retrieval and incuabate overnight in refrig. (use at 1:50,000). I
use Signet's ultra streptavidin (USA) detection and AEC for the
chromogen. Let me know if you would like more information.
Pam
On Tue, 11 Jul 2000, Jenny Molde wrote:
> Dear Histonetters
> I am looking for a lymphocyte marker for sheep. Anyone out there had any
> experience with this. Many thanks.
>
> Jenny Molde
>
>
>
>
Pam Dilbeck
Immunohistochemistry Section
Washington Animal Disease Diagnostic Laboratory
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