Re: Lillie's ferric ferricyanide reduction reaction for ferric ions.

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:Victoria Baker <>
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Victoria Baker wrote:

> Hi!
> I have another oldie, but goodie.  Lillie devised a
> stain back in 1965 for ferric ions, we are currently
> looking to use this stain to identify porphyria in
> liver.  I was wondering if anyone had a copy of this
> procedure available.  I was given a paper written back
> in 1997 that has part of the procedure, but not enough
> to fully prepare a protocol for it.
> As always, thanks for any assistance anyone may have.
> Vikki Baker
> American Health Foundation
> Valhalla, New York

The stain for ferric iron is the Prussian Blue reaction, a histochemical
reaction dating to the 19th century.
1. Bring paraffin sections to water.
2. Incubate 30 minutes in a mixture of equal parts of 2% potassuim
ferrocyanide and 2% concentrated HCl. Mix this solution IMMEDIATELY
before use.
3. Rinse in distilled water.
4. Counterstain lightly with a red nuclear stain.
5. Dehydrate, clear and mount.

Ferric iron deposits will be blue-green.

If you want to detect ferrous iron (the Turnbull blue rxn.) substitute
potassium ferricyanide above.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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