I Lynn,
I'm learning more and more about In situ Hyb. daily, so I am no 
expert by any stretch. I hadn't thought of the waterbath as a source 
of RNase contamination until you mentioned it, but is it possible that 
the RNA in the tissue is protected buy the hydrophobic nature of the 
paraffin?? If we can't find out for sure, I guess using DEPC treated 
water wouldn't be too much of a hardship! Looking forward to 
following this one up. Hope all replies are posted for all to see! 
Cheers!  Greg

Date sent:      	Thu, 29 Jun 2000 18:13:12 -0500
From:           	Lynn Gardner <lynn-gardner@uiowa.edu>
Subject:        	Insitu Paraffin
Forwarded to:   	DOBBIN@acad1.cs.upei.ca
To:             	Histonet@pathology.swmed.edu

> Dear histonetters,
> 
> I would be most interested in finding someone out there who does Insitu on
> Paraffin sections routinely and who has it running consistently. I would
> like to find out if that person or persons would be willing to share their
> procedure with us as we would like to get it started here. We already do
> Insitu on Frozens but would like to work with paraffin. 
> 
> Any help is greatly appreciated. Please either e-mail the information or
> feel free to fax it to me at 319-335-7770.
> 
> Thanks everyone!
> 
> Sincerely,
> Lynn Gardner, HT(ASCP)
> 
> 


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>
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851