Re: Immunos on Cytology Specimens

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From:Dana Settembre <settembr@UMDNJ.EDU>
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On Thu, 6 Jul 2000 wrote:

> I would appreciate any feed back on Immunohisto procedures done on
> cytology smears,.cytospins etc.
> We recently purchased a Biogenex Optimax Immuno stainer (and love it).
> I heard that you could fix smears etc. in cold acetone and then start
> your normal run procedure. Has any one used anything else and had great
> results?
> Thanks
> Deb Ritter, HT ASCP
> St. Marys Hosp.
> Grand Junction, Colorado

Hello Deb,
When I run cytology smears I start them at buffer and join them with my
regular paraffins at the H2O2 step.

When I get them, usually I get them in water, I put them in buffer.  In
the mean time my paraffins are deparaffinzing to water and getting
pretreated as necessary and go into buffer and H2O2 with the smears.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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