Re: Immuno EM methods

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet)
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Joyce Kotzuk wrote:

> My P.I. is interested in trying immunohistochemistry on tissue that has
>been prepared for EM, even tissue that is already embedded in plastic or
>resin or whatever.  I have never done EM, nor worked with (ie. cut) tissue
>in plastic.  Could someone suggest some references or a protocol for doing
>immuno on tissue such as this?  I got the references about antigen
>retrieval in EM tissue that were recently posted on the Histonet, but I
>need basic methods, and how they differ from regular immuno. Thanks in
> Joyce Kotzuk, Univ. of New Mexico pathology dept.


    There a lot of variables here! What antigen? What resin is the tissue
in? Spurr is quite " anti-immuno". What fixation? How long? If osmium was
involved you may have to forget it and start with fresh tissue. Sorry!

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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