Joyce Kotzuk wrote:

> My P.I. is interested in trying immunohistochemistry on tissue that has
>been prepared for EM, even tissue that is already embedded in plastic or
>resin or whatever.  I have never done EM, nor worked with (ie. cut) tissue
>in plastic.  Could someone suggest some references or a protocol for doing
>immuno on tissue such as this?  I got the references about antigen
>retrieval in EM tissue that were recently posted on the Histonet, but I
>need basic methods, and how they differ from regular immuno. Thanks in
>advance,
> Joyce Kotzuk, Univ. of New Mexico pathology dept.
> jkotzuk@salud.unm.edu

Joyce:

    There a lot of variables here! What antigen? What resin is the tissue
in? Spurr is quite " anti-immuno". What fixation? How long? If osmium was
involved you may have to forget it and start with fresh tissue. Sorry!

Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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