Re: CJD prion antibody from Dako

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From:Tina Cardamone <>
To:Vinnie Della Speranza <>
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Dear Vinnie,

I use a monoclonal antibody called 3F4 from Senetek.
Purified IgG 1.0mg/ml code 620-02.
I use it at 1:0000. It needs autoclave antigen retrieval for 30 mins.
It is a great antibody and I have never had any problems.
Senetek ,PLC
620 Airpark rd.
Napa, CA 94558
Tel, (707)226 3900
Fax, (707)226 3999

I hope this helps.
Good Luck

Kind Regards,
Tina Cardamone
University of Melbourne

At 03:25  6/07/00 -0400, you wrote:
>I would be interested in hearing from anyone who has achieved successful 
>immunohistochemical staining in formalin fixed, paraffin embedded tissues 
>with Dako's antibody to the Creuzfedt-Jakob prion.
>Our attempts with this product have been unsuccessful thus far.
>if someone knows of a similar antibody that works well, would you be kind 
>enough to pass this information along?
>Vinnie Della Speranza
>Manager for Anatomic Pathology Services
>Medical University of South Carolina
>165 Ashley Avenue
>Suite 309
>Charleston, SC  29425
>ph:  (843) 792-6353
>fax: (843) 792-8974

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