Re: B5 Fixative

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From:Becky Scholes <>,
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We switched from the hazardous B-5 to Anapath's alcoholic zinc formalin.  It 
works pretty well, except you need to fix the grossed tissue for at least 
one hour in formalin before transferring it to the AZF.  We fix in the AZF 
then for at least one hour, maximum eight hours.  I'm at home, so I don't 
have the phone number or catalog number.  Hopefully you already have the 
phone number.  If not, I can bring home the info to post.

Bec the Tec

>From: "Karnes, Lori  SDH" <>
>To: "''" <>
>Subject: B5 Fixative
>Date: Wed, 12 Jul 2000 12:41:09 -0600
>  In our Histology Lab we have been fixing our bone marrows and fresh lymph
>nodes in B5 fixative. We have recently changed our BM so they are now fixed
>in IBF from Surgipath. I was wondering if this fixative is also OK for 
>lymph nodes. I would also like to know if a comparison was done on these
>specimens. I would like to stop using B5 if at all possible as it is such a
>health hazard. We also fix our liver biopsies in B5. Any comments would be
>greatly appreciated. Thanks in advance.
>Lori Karnes ART
>Histology Lab
>Royal University Hospital
>Saskatoon, Sask.

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