Re: B5 Fixative

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From:Victor Tobias <>
To:"Karnes, Lori SDH" <>
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We are in the beginning stages of doing a blind study of B-5 substitutes. This
will take awhile to complete and get feedback from all the pathologists. We will
be sampling BM and liver.

Victor Tobias
Histology Supervisor
University of Washington Medical Center
Seattle, WA

"Karnes, Lori SDH" wrote:

>  In our Histology Lab we have been fixing our bone marrows and fresh lymph
> nodes in B5 fixative. We have recently changed our BM so they are now fixed
> in IBF from Surgipath. I was wondering if this fixative is also OK for fresh
> lymph nodes. I would also like to know if a comparison was done on these
> specimens. I would like to stop using B5 if at all possible as it is such a
> health hazard. We also fix our liver biopsies in B5. Any comments would be
> greatly appreciated. Thanks in advance.
> Lori Karnes ART
> Histology Lab
> Royal University Hospital
> Saskatoon, Sask.

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