Re: Alcian blue - H & E

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From:"J. A. Kiernan" <>
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On Mon, 3 Jul 2000 wrote:

> Is anyone familiar with doing an Alcian Blue pH 2.5-H&E stain in place of an 
> H & E to see goblet cells?

  Alcian blue at pH 2.5 stains the contents of intestinal goblet
  cells a pleasing turquoise-blue. This colour is not extracted by
  later washing, staining, dehydrating etc. This is the tip of the
  iceburg of carbohydrate histochemistry with dyes. 

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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