Re: Alcian blue - H & E
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | CMD1352@aol.com |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 3 Jul 2000 CMD1352@aol.com wrote:
> Is anyone familiar with doing an Alcian Blue pH 2.5-H&E stain in place of an
> H & E to see goblet cells?
Alcian blue at pH 2.5 stains the contents of intestinal goblet
cells a pleasing turquoise-blue. This colour is not extracted by
later washing, staining, dehydrating etc. This is the tip of the
iceburg of carbohydrate histochemistry with dyes.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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