Re: AEC alternative
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From: | "Brothers, Rachel" <rachel_stock@internet.organo.com> |
To: | n.coulston@unsw.edu.au, histonet@pathology.swmed.edu |
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Content-Type: | text/plain; charset=US-ASCII |
Neralie,
>I was wondering whether there are any good alternatives to AEC as a
>substrate for peroxidase when used in immunohistochemistry on
>paraffin sections.
I've started using Vector's NovaRed chromogen in place of AEC and have
gotten satisfactory results. You get the red color with the advantage
that it can go through regular ethanol dehydration/clearing steps
without disappearing.
Best regards,
Rachel Stock HTL(ASCP)
Organogenesis, Inc.
150 Dan Road
Canton, MA 02021
(781)575-0775
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