RE: frosty cryostat
<< Previous Message | Next Message >>
From: | jim <jim@proscitech.com.au> |
To: | "'Valleygal@aol.com'" <Valleygal@aol.com>, "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Here are a couple of answers:
Refrigerated air-conditioning lowers room humidity. If you have controls, then
use recirculate (recycling more dehumidified air) and low fan speed (less
efficient cooling but more efficient moisture collection) settings for better
dehumidification.
Moisture condenses on the coldest surface, which is of course, your cryostat.
If you have ready access to liquid nitrogen or dry ice, place some on / in a
suitable uninsulated container within the cryostat. A sizeable metal container
would be most efficient in collecting moisture, but don't burn yourself on the
cold metal.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com
On Sunday, July 02, 2000 2:51 AM, Valleygal@aol.com [SMTP:Valleygal@aol.com]
wrote:
> Fellow Histonetters,
> This question is directed mostly to those techs who live in humid areas and
> do lots of frozens in old labs which are not "climate controlled".
> Usually Tucson has a very dry climate but during the summer monsoons it gets
> a little swampy. We are in the monsoon season now and the rain is great for
> most things but not for my cryostat.
> My cryostat, a Leica 2800N, has been frosting up something awful because it
> is so humid in our room. I put a tray of drierite in the chamber and it
> turned pink almost immediately. Of course it doesn't help to be cutting
> sections and having the window open for long stretches of time. Does anybody
> have any tips on preventing the frost build up in a normally frost free
> cryostat?
> Thanks!
> Andi Grantham
> University of Arizona
>
> **********************************************************************
> =^..^=
<< Previous Message | Next Message >>