How about immunohistochemistry? You could use GFAP antibody for the glial
cells and neurofilament or other neuronal marker for the neurons. 
Let me know if you need help.



Lilith
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Lilith Ohannessian-Barry                               
National Research Council                          
Institute of Biological Sciences                  
CANADA
Tel;613-993-6460
Fax;613-941-4475
e-mail; lilith.barry@nrc.ca









-----Original Message-----
From: Candace Andersson [mailto:candace.andersson@css.unc.edu]
Sent: Wednesday, July 12, 2000 10:24 AM
To: histonet@pathology.swmed.edu
Subject: differential neuronal/glial stain


I was wondering if anyone has any knowledge of a staining procedure which
differentially stains neurons and glial cells in cerebral cortex.  I have
20 um frozen, cryostat sections of rat prefrontal cortex that have been
perfused with 2% paraformaldehyde.  I recently came across a reference
from 1968 whichdescribes a "hematoxylin lake modification" of a Grimley,
Albrecht and Micheltich procedure which uses Grimley's malachite green
solution and basic fuchsin solution, but I would appreciate any further
information.
I need a straightforward staining procedure.  We are staining this tissue
for 
sterological analysis and are trying not to shrink the tissue by more than 
50%.

Randal Brown
Dept. of Psychiatry
University of North Carolina, Chapel Hill