RE: avidin/biotin block for ihc

<< Previous Message | Next Message >>
From:Cynthia Favara <>
To:"''" <>,
Content-Type:text/plain; charset=iso-8859-1

This is from a course given by RT Miller in 1998. i have used when necessary
and found it to be very effective,
Avidin block:
	2 egg whites diluted to 200ml with distilled water for 15 minutes at
room temperature. Before this step slides should be thoroughly rinsd in
distilled water as salt solutions[PBS] may precipitate the proteins in the
egg whites. Rinse well with distille water to remove the somewhat tenacious
egg white. immerse in 0.2% biotin [ICN] in PBS 15 minutes at
roomtemperature. Hope this helps.
Cynthia Favara 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: <> 


<< Previous Message | Next Message >>