RE: Weigert's Elastic Tissue Stain

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From:Mark Ray <darkdaym@earthlink.net>
To:Histonet <Histonet@pathology.swmed.edu>, "J. A. Kiernan" <jkiernan@julian.uwo.ca>
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Thanks, John.  I see that my unwitting, mercenary haste precipitated an
avalanche of misunderstanding.  I hope that everyone whom I may have misled
will read your message.

Mark  


> [Original Message]
> From: J. A. Kiernan <jkiernan@julian.uwo.ca>
> To: Histonet <Histonet@pathology.swmed.edu>
> Date: 7/1/00 6:26:54 PM
> Subject: RE: Weigert's Elastic Tissue Stain
>
> On Thu, 29 Jun 2000, Mark Ray wrote:
> >  EK Industries lists Order Number 9745-500ML  
> >  VERHOEFF-VAN GIESON ELASTIC TISSUE STAIN KIT
> and there were many similar replies to the question.
> 
> Weigert and Verhoeff were different people.
> 
> Weigert's stain for elastic fibres and laminae is based on the
> use of a dye formed by heating basic fuchsine, resorcinol and
> ferric chloride. This is traditionally done in the lab, but
> pre-made "resorcin-fuchsin" is also an article of commerce.
> Weigert's method is quite different from Verhoeff's iron-iodine-
> haematoxylin, which is easy to make and use, but also stains 
> nuclei and myelin.  
> 
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1
> 
> 
> 



--- Mark Ray
--- darkdaym@earthlink.net
--- EarthLink: It's your Internet.





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