We are using 34Be12 from Nova Castra.  We use Protease 1 for 16 minutes and
the antibody for 32 minutes.  We get very nice staining of our prostate
tissue.

jennifer macdonald

> ----------
> From: 	Sanders, Julie, VHACIN[SMTP:Julie.Sanders@med.va.gov]
> Sent: 	Friday, July 14, 2000 7:30 AM
> To: 	'Histonet@pathology.swmed.edu'
> Subject: 	TTF-1 and CKBeta34E
> 
> Good Morning All,
>    We are having problems with the TTF-1 on the Ventana.  I have spoken to
> the vendor of the antibody, and we have tried everything under the
> sun...longer antigen retrieval time, using BioCares Nuclear Decloaker,
> different TTF-1 from other vendors (all prediluted) to no avail...any
> suggestions?
>    Also, we do CKBeta34E on prostate, but prostate is not working as a
> control.  Skin works wonderfully, but our pathologists insist that we use
> a
> prostate control...again suggestions will be welcomed. 
> 
> Thanks in advance for the responses,
> Julie
> 
> Julie A. Sanders, BA, HT(ASCP)
> Supervisor, Anatomic Pathology/Administrative Office
> VAMC, Cincinnati, Ohio
>