RE: Staining blood cells in blood

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From:"MacDonald, Jennifer" <jmacdonald@sach.org> (by way of histonet)
To:histonet@histosearch.com
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If this is the case it is done for reticulocytes.  In vitro staining.  Drops
of anticoagulated blood are added to a staining solution and incubated.
After the incubation a film is made.  This film is dried and examined under
oil immersion to count the reticulocytes.  I don't see why you couldn't
examine the cells wet under a coverslip.

Jennifer MacDonald



> I want to make sure I am readling this correctly.  Are you trying to stain
> the
> blood before you make the thin films?
>
> Rande
>
>
>
>
> Richard Levenson <rml52@yahoo.com> on 07/05/2000 04:33:23 AM
>
> To:   histonet@pathology.swmed.edu
> cc:    (bcc: Rande Kline/EMI/Merck)
> Subject:  Staining blood cells in blood
>
>
>
> Dear Histonetters:
>
> I am trying to set up a system that can take whole blood (anticoagulated)
> and add a stain such as a Giemsa or Wright stain (or a compatible
> combination of stains) and then make a thin wet film without drying or
> washing the cells. (A homogeneous assay, minimal processing steps).
>
> The desired outcome would be that the cells concentrate the stain and
> ideally exhibit some kind of metachromatic behavior, with preservation of
> cell morphology, and little interference from the dye surrounding the
> cells.
> It would be acceptable to have more than one dye in the mixture, as long
> as
> that added to the discriminating power. I realize that some stains require
> a
> differentiating step (addition of water, perhaps), but I'm trying to avoid
> anything other than the step of adding a stain (plus buffer, alcohol,
> detergent as needed). The goal is to be able to identify WBCs using a
> combination of staining behavior and size and shape parameters.
>
> Thanks,
>
> Richard
> --
> Richard Levenson, MD
> Technical Director, Biomedical Systems
> CRI, Inc.
> 80 Ashford St.,
> Boston, MA 02134
> rlevenson@cri-inc.com
>
>
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