RE: Slightly OT: Live cells anyone?

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From:Cynthia Favara <>
To:'Jerry Chi-Yuan Hu' <>,
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	You do not have to embedd to cut frozen sections. I am presuming
[always dangerous] you are working with a piece of tissue. Tissue can be
flash frozen in liguid nitrogen and can then be put on a chuck for cutting.
It is possible to do this so the tissue remains frozen by dipping the chuck
in liquid nitrogen and slowly building a little throne for the tissue. Make
sure the tissue remains frozen as well. 100um pieces may crack but since you
are going to isolate cells I would not think cracking would matter.
Good Luck,
Cynthia Favara 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: <> 


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