Jerry,
	You do not have to embedd to cut frozen sections. I am presuming
[always dangerous] you are working with a piece of tissue. Tissue can be
flash frozen in liguid nitrogen and can then be put on a chuck for cutting.
It is possible to do this so the tissue remains frozen by dipping the chuck
in liquid nitrogen and slowly building a little throne for the tissue. Make
sure the tissue remains frozen as well. 100um pieces may crack but since you
are going to isolate cells I would not think cracking would matter.
Good Luck,
Cynthia Favara 
NIAID/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov>