RE: Slightly OT: Live cells anyone?

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From:jim <>
To:'Jerry Chi-Yuan Hu' <>
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Easy. You need a Vibratome. These have a vibrating blade and this means cutting 
can occur without compression. You can look at our online pages, but we cannot 
sell into the N American market.
Jim Darley

ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560            

On Tuesday, July 11, 2000 10:53 PM, Jerry Chi-Yuan Hu 
[] wrote:
> Hello Everyone,
> I am looking for a way to isolate cells from thin slices (at 100 um
> thick) and to start a cell line from these cells.  Everyone I've spoken to
> says that the fresh tissue has to be embedded for slicing in a microtome
> cryostat, though slices of 100 um might crack...  I also do not know if
> the embedding medium is going to kill my cells.
> If anyone knows how to use the microtome (or cryotome) to get thick slices
> of live cells please let me know.  Your help is greatly appreciated!
> Jerry Hu

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